Usually, the sample is collected through ejaculation into a sterile collection container. The semen is to be delivered within a half hour of ejaculation, so if one lives close enough the sample can be collected at home. If not, one has to collect a sample in the clinic in a special room.
There is a delay between when the semen sample is dropped off for washing and when it is inseminated. The amount of time depends on the washing technique used, which takes 1 to 2 hours.
Ideally, an IUI is done within a few hours of the documentation of ovulation. When timing is based on an hCG injection, the IUIs are usually done between 36-42 hours post hCG trigger. Ideal timing would be to have a single IUI at about 36 hours post-hCG. If two IUIs are scheduled, they are usually done at 24 hours and 48 hours post hCG to cover the period of ovulation.
The success rate depends upon the development of one or multiple follicles and also upon sperm count and motility. Overall success rate seems to be 10-15percent per cycle.
Most of the IUI is painless. Some women experience cramps due to procedure or ovulation.
Current research indicates that washed sperm can live up to 72 hours also; however, it does lose fertility power to a certain extent after 24 hours.
Post IUI 15-20 minutes rest is given after which you can resume your routine work.